Metabolomix+ : A Non-Invasive Personalized Nutritional Assessment

The Metabolomix+ is a unique combination of nutritional tests that provides an analysis of key nutritional biomarkers. A first morning void (FMV) urine collection, with optional add-on bloodspot finger stick and buccal swab, the Metabolomix+ nutritional test is a non-invasive, patient-friendly way to assess the functional need for antioxidants, B-vitamins, minerals, digestive support, fatty acids, and amino acids. Insights gained from the Metabolomix+ nutritional test allows clinicians to target nutritional therapies to meet the precise needs of their patients.

Metabolomix+ is one of the most comprehensive functional and nutritional assessments available. It is designed to help practitioners identify root causes of dysfunction and provide a systems-based approach to help patients overcome chronic conditions and live a healthier life.

The Metabolomix+ report offers:

  • Nutrient recommendations for key vitamins, minerals, amino acids, fatty acids, and digestive support
  • Functional pillars with a built-in scoring system to guide therapy around needs for methylation support, toxic exposures, mitochondrial dysfunction, fatty acid imbalances, and oxidative stress
  • Interpretation-At-A-Glance pages for patient education
  • Dynamic biochemical pathway charts for clearer understanding

What is a functional nutritional assessment?

Marked accumulation of organic acids in urine can signal a metabolic inhibition or block. The metabolic block may be due to a nutrient deficiency, an inherited enzyme deficit, toxic build-up, or drug effect.

Enzymes that are responsible for metabolizing organic acids are vitamin and mineral dependent. With this, elevations in organic acids can reflect a functional need for these nutrients on a cellular and biochemical level, even despite normal serum levels.1-5 Recommendations for nutrient supplementation based on elevated organic acid results are generated using a literature-based proprietary algorithm.

Traditionally, urinary organic acid assessment has been used in neonatal/pediatric medicine to identify genetic inborn errors of metabolism, with severity depending on the degree and type of error.* In many cases of genetic inborn errors, the enzymatic defect may be compensated for by high doses of specific vitamin and mineral cofactors and/or dietary interventions. Intervention with higher-dose nutrient cofactors may also be effective in cases of decreased enzyme activity due to causes other than frank inborn errors.

* Genova’s organic acid testing is not intended for the diagnosis of neonatal inborn errors of metabolism.

Which patients might benefit from functional nutritional testing?

Common clinical indications for testing include:

  • Mood Disorders6,7
  • Mitochondrial Dysfunction8
  • Fatigue9
  • Chronic Stress10
  • Inflammation11

Several diseases are associated with abnormal organic acid, amino acid, and fatty acid levels such as depression, anxiety, cardiovascular disease, neurocognitive decline, diabetes, cancer, anorexia, and many others.12-15

About the Metabolomix+ Profile

The Metabolomix+ Profile report allows for easy interpretation and clinically actionable results. It includes a Suggested Supplement Schedule that provides personalized recommendations based on test results. The Interpretation-At-A-Glance section of the report provides facts related to nutrient function, causes and complications of their deficiencies, and dietary sources.

The Metabolomix+ Profile report categorizes results into several metabolic areas:


  • Organic Acids (urine)
  • Malabsorption and Dysbiosis Markers are metabolites produced by the gastrointestinal microbiome
Analyte List
2-Hydroxyphenylacetic Acid
3-Hydroxyproprionic Acid
4-Hydroxyphenylpyruvic Acid
5-OH-Indoleacetic Acid
APOE (Add-on)
Adipic Acid
Alpha-amino-N-butyric acid
Alpha-aminoadipic acid
Aluminum (Add-on)
Anserine (dipeptide)
Antimony (Add-on)
Arachidic (Add-on)
Arachidonic (Add-on)
Arsenic (Add-on)
Aspartic acid
Barium (Add-on)
Behenic (Add-on)
Benzoic/Hippuric Acids Ratio
Beta-aminoisobutyric acid
Bismuth (Add-on)
COMT (V158M) (Add-on)
Cadmium (Add-on)
Calcium (Add-on)
Carnosine (dipeptide)
Cesium (Add-on)
Chromium (Add-on)
Cis-Aconitic Acid
Citramalic Acid
Citric Acid
Cobalt (Add-on)
Copper (Add-on)
Creatinine (Add-on)
Dihomo-gamma-linolenic (Add-on)
Dihydroxyphenylpropionic Acid
Docosahexaenoic (Add-on)
Docosapentaenoic (Add-on)
Docosatetraenoic (Add-on)
Eicosadienoic (Add-on)
Eicosapentaenoic (Add-on)
Elaidic (Add-on)
Formiminoglutamic Acid (FIGlu)
Gadolinium (Add-on)
Gallium (Add-on)
Gamma-Linolenic (Add-on)
Gamma-aminobutyric acid
Glutamic acid
Glutaric Acid
Glyceric Acid
Glycolic Acid
Homovanillic Acid
Indoleacetic Acid
Iron (Add-on)
Isocitric Acid
Kynurenic Acid
Lactic Acid
Lead (Add-on)
Lignoceric (Add-on)
Linoleic (Add-on)
Lipid Peroxides
Lithium (Add-on)
MTHFR (C677T and A1298C) (Add-on)
Magnesium (Add-on)
Malic Acid
Manganese (Add-on)
Margaric (Add-on)
Mercury (Add-on)
Methylmalonic Acid
Molybdenum (Add-on)
Nervonic (Add-on)
Nickel (Add-on)
Niobium (Add-on)
Oleic (Add-on)
Orotic Acid
Oxalic Acid
Palmitic (Add-on)
Palmitoleic (Add-on)
Pentadecanoic (Add-on)
Phenylacetic Acid
Platinum (Add-on)
Potassium (Add-on)
Pyroglutamic Acid
Pyruvic Acid
Rubidium (Add-on)
Selenium (Add-on)
Stearic (Add-on)
Strontium (Add-on)
Suberic Acid
Succinic Acid
Sulfur (Add-on)
TNF-a (Add-on)
Thallium (Add-on)
Thorium (Add-on)
Tricosanoic (Add-on)
Tungsten (Add-on)
Uranium (Add-on)
Vaccenic (Add-on)
Vanadium (Add-on)
Vanilmandelic Acid
Zinc (Add-on)
a-Keto-b-Methylvaleric Acid
a-Ketoadipic Acid
a-Ketoglutaric Acid
a-Ketoisocaproic Acid
a-Ketoisovaleric Acid
alpha-Linolenic (Add-on)
b-OH-Butyric Acid
b-OH-b-Methylglutaric Acid
CPT Codes
1-Methylhistidine 82139
2-Hydroxyphenylacetic Acid 83921
3-Hydroxyproprionic Acid 83921
4-Hydroxyphenylpyruvic Acid 83921
5-OH-Indoleacetic Acid 83497
8-OHdG 83520
Adipic Acid 83921
Aluminum (Add-on) 82108
Antimony (Add-on) 83015
Arachidic (Add-on) 82542
Behenic (Add-on) 82726
Cadmium (Add-on) 82300
Calcium (Add-on) 82340
Citric Acid 82507
Creatinine 82570
D-Arabinitol 84311
Docosatetraenoic (Add-on) 82726
Glutaric Acid 83921
Glyceric Acid 83945
Glycolic Acid 83945
Homovanillic Acid 83150
Isocitric Acid 83921
Lactic Acid 83605
Lead (Add-on) 83655
Lignoceric (Add-on) 82726
Lipid Peroxides 84311
Magnesium (Add-on) 83735
Malic Acid 83921
Methylmalonic Acid 83921
Nervonic (Add-on) 82726
Nickel (Add-on) 83885
Orotic Acid 83921
Oxalic Acid 83945
Pyruvic Acid 84210
Suberic Acid 83921
Succinic Acid 83921
Tricosanoic (Add-on) 82726
Vanilmandelic Acid 84585
a-Hydroxybutyrate 83921
a-Keto-b-Methylvaleric Acid 83921
a-Ketoadipic Acid 83921
a-Ketoglutaric Acid 83921
a-Ketoisocaproic Acid 83921
a-Ketoisovaleric Acid 83921
Specimen Requirements
1 green-top preservative tube - FMV urine (frozen); 1 blue-top amber tube - FMV urine (frozen); 1 green-top tube - FMV urine (frozen); Additional add-ons include 1 bloodspot card; 2 genomic buccal swabs


Additional Resources