One Day Hormone Check™


A 24-hr Salivary Hormone Test for Assessing Key Hormones

The One Day Hormone Check™ is a convenient salivary hormone test that evaluates unbound, bioavailable hormone levels over 24 hours. Specimens collected over 24 hours are examined for levels of estradiol, estrone, estriol, progesterone, the progesterone/estradiol ratio (P/E2), testosterone, cortisol, DHEA, and melatonin.

The One Day Hormone Check provides information about the impact that shifting hormone levels can have in men (andropause or male menopause) and women (perimenopause and menopause). It can reveal imbalances of primary sex hormones and how they relate to other hormones, such as DHEA, cortisol, and melatonin. Using hormone testing, clinicians can customize hormone and/or nutritional therapies and monitor therapy.

Patients with hormone imbalance may present with:

  • Fatigue
  • Weight loss or gain
  • Menstrual irregularities
  • Loss of libido
  • Insomnia
  • Changes in hair and skin
  • Hot flashes

The One Day Hormone Check salivary assessment offers several advantages for patients presenting with hormone-related symptoms:

  • Comprehensive testing: five sex hormone saliva samples collected over a 24-hour period provide the level of estrone (E1), estradiol (E2), estriol (E3), progesterone, the progesterone/estradiol ratio (P/E2), testosterone. Cortisol, DHEA, and melatonin are also measured.
  • Reporting: reference and therapeutic ranges enable the physician to choose the optimal reference cohort for their patient
  • Patient ease-of-use: saliva collection is a simple, non-invasive, at-home collection. It is ideal when multiple samples are required to be collected over a period of time

To enhance clinical utility, a therapeutic cohort results cover page is provided for the clinician to quickly see where patient results fall in comparison to a cohort of peri/menopausal women treated with bioidentical hormone therapy.

Optional Add-on:

  • Cortisol Awakening Response (CAR)
  • Two awakening samples to evaluate CAR
  • CAR is a transient, immediate rise in cortisol upon awakening and is distinct from the diurnal rhythm. CAR reflects a person’s ability to cope with anticipated challenges and their perception of control around chronic stress, providing insight into HPA axis resiliency.

Test Type: Saliva Test

Analyte List
CPT Codes
Cortisol 82530
DHEA 82626
Estradiol 82670
Estriol 82677
Estrone 82679
Melatonin 83520
Progesterone 84144
Testosterone 84402
Specimen Requirements
5ml saliva


Additional Resources